Genotyping specific genetic markers (loci),
has become a standard of many population and pharamacogenomic
studies. Typically, this is accomplished using variable
number tandem repeats (VNTR), short tandem repeats (STR),
restriction fragment length polymorphism (RFLP, and single
nucleotide polymorhisms (SNP). Each of these methods of
identification has its strengths  and
weaknesses. The two methods that we have found extremely
helpful to the scientific community, largely based on cost,
informativeness and automatability are STR and SNP testing.
STR has been shown to be particularly useful for population
genetic studies and has been widely used for identification
of convicted felons through the FBI’s combined DNA
indexing system (CODIS) database. SNP testing in also has
its place in population genetics testing particularly for
deep ancestral origin testing, however, SNP’s are
extremely powerful in disease diagnostics and pharmacogenomic
studies.
Some of our preferred methods are shown below.
Product pricing varies depending on the size and scope of
the project.
STR Genotyping
Tap into our sets of STR population multiplex
panels for:
• Y chromosome – 43 STR markers
• X chromosome – 14 STR markers
• Core autosomal STR’s – 15 STR markers
(13 from the core CODIS FBI set) plus two additional markers
recommended for the UK and the Amelogenin
• Extended autosomal panels – includes more
than 100 linked and unlinked autosomal markers.
Custom STR marker and panel development.
Based on the needs of the client and the
scope of their project, Sorenson Genomics will:
• Research the project requests
• Designs STR primers to fit the project
• Order STR primers
• Validate PCR run parameters using basic thermocycling
methods
• Produce validation run data from a standard population
sample set using ABI capillary DNA analyzers. ABI 3100
is recommended for low volume project and ABI3730XL is
recommended for high volume projects.
Custom SNP marker and panel development.
Based on the needs of the client and the
scope of their project, Sorenson Genomics will:
• Research the project requests
• Designs SNP primers to fit the project
• Order SNP primers in multiplexes up to 48 primer
sets per reaction
• Validate PCR run parameters using ABI SNPlex test
kit
• Produce validation run data from a standard population
sample set using ABI3730XL capillary DNA analyzer.
Sorenson Genomics can also provide assistance
if your SNP project is smaller scale i.e. less than 10 SNP’s.
This may be applicable for diagnostic assay development.
Data from the above projects can be posted
to a secure FTP site or e-mailed as a raw electropherogram
file. We can also assist clients with database and statistical
analysis upon request.
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