Sorenson Genomics has developed extensive experience in the area of DNA extraction and purification. This experience comes from years of testing and validating different DNA extraction methods to ensure recovery of DNA that is of the highest quality and quantity. Our DNA extraction experience ranges from recovering DNA from highly degraded bone and teeth fragments to processing high volume blood, mouthwash or buccal cell extractions for the purpose of clinical or pharmacogenomic trials. Some of our preferred methods are shown below. Product pricing varies depending on the size and scope of the project.

DNA extraction, purification, qualification and quantification.

Specimen types routinely tested:

• Buccal swabs
• Whole blood
• Blood spots on FTA paper
• Mouthwash
• Tissue
• Plasmid isolates
• Forensic specimens including hair, bone, teeth etc. often associated with missing persons or disaster victim identification.

Methods of DNA extraction and purification:

• QIAGEN robotic HT blood or buccal cell.
• QIAGEN manual large volume blood.
• Gentra Autopure large volume blood or mouthwash.
• QIAGEN EZ-1 forensic specimen extraction.

DNA purity and quantitation analysis:

• Standard spectrophotometry
• Low volume spectrophotometry (2 -10 uL input volume)
• Fluorometric analysis using pico green.

Purified DNA can be provided to our clients in cryovial tube, standard 96 well microtiter plates, .5 mL deep well 96 well plates and 1.2 mL deep well 96 well plates. DNA can be normalized to a specific concentration to meet the needs of the client’s particular project.

Next: DNA Analysis